Beiträge von Steve_mt

We are going for it. Hope we find it in goid shape 🙏😊

You hit the nail on its head re last fungus. I see if I can do more re species level! Interesting!

Thanks!

Ceratellopsis

But spores, bacteria and living matter that have water inside so the microwave radiation "should" destroy them, at least theoretically. The question is if the rating of 440W is enough, because higher ratings damage plastic, wood/paper, rubber and arcs with metals etc.

Microwave sterilization - PubMed

Effect of microwave radiation on Bacillus subtilis spores - PubMed

Yet these claims do not mention the power rating So I wonder if someone had personal experience.

Wish to have confirmation on my ID of Mycena hiemalis for this wood-inhabiting mycena. Saprotrophic on Ceratonia siliqua

I know that microwave should kill all living matter. When it comes to plastic or paper or metals, setting at high-power can cause burns and damages to such materials, but at 270W and 330 W (I think also 440W) no damage is created. Do you know if at these ratings for long period (say 10mins) is an effective way of killing bacteria and fungi spores. Sometimes I re-use petridishes coz they are expensive.

Guys, for the last 4-5 years I have been trying to identify a Talaromyces that specifically grow on seeds of Washingtonia.

You can follow and see the pics here:

https://mushroomobserver.org/262509

To sequence it, I have to isolate it on media, which I managed. Now I need to dry it. I have a home made incubator with temperature control (now set at 40C). My plan is to put the petri dish in it but the water moisture evaporating out will condense on the lid and back into the media. I thought of uncovering it but I am afraid it gets contaminated leaving it uncovered for a day. Ideas:

Drilling the lid with small hole / holes (less chance of contamination)

Place a sterile filter paper instead of the lid.

Sprinkle over sulfur to inhibit growth of any fungi (just a weird idea!).

Put petri dish it in a sterile, close tupperware container with the lid open over a bed of dry silica gel

How should I dry it without contaminating it.

The specimens started to mold slightly :-(. Yesterday I did not put them in the fridge, so as to accelerate the development process. The best specimen has been dried yesterday so safe from contaminants. Today, I thought, dass die surface was more pruinose ... maybe they make it to sporulate before parasite takes them out.

Andreas, KOH on surface - no color change. It wasn't gelatinous (mucoid or watery) just soft tissue

Yet I found some other strange finger-like organism growing close to them from the soil. Maybe Ceratiomyxa ?!. I may find time to examine under the microscope!

Dear Pablo,

Thank you for your reply and teaching. I could clearly make up two types of hyphae and maybe the third one, the binding hyphae (not sure) as shown in the new image here (Sorry for the crap ones before, I was super tired!)

Thanks a lot for yr help

LG

Stephen

OK I have a small update. I re-examined the material which was in fridge for 3 days. They have not changed much physically, but the outer layer became slightly more developed and we can be sure that this is a Basidiomycete since I saw basidioles. None of them was mature. The medull became completely whitish-yellow now (central orange zone faded away) and there were some invaginations inside (like it is still shaping up). The medullary tissue remains undifferentiated, in parallel bundles, but they are elongated, more straight and still with perpendicular septae (- rectangular hyphae)

I attach the images.

Thanks for suggestion Spathularia Grüni /Kagi ... hmmmm, could be but they are mostly yellow. I have another suggestion - what do you think about Dacryopanix???

Yes, I did. I saw a tobacco brown trama which with 4% KOH it becomes wine-red, darkening to blackish-purple. I tried to mount the trauma and tubes and I am sure that there are two types of hyphae and maybe three (trimitic) if the basidiole baring hyphae are considered different from the generative. The hairs are two types, brown and dull brown (almost black). The hymenium have a lilac tinge at the border.

Spores really lemon-shaped : 15.6 x 9.3 µm; Qe = 1.7; Ve = 721 µm3

- hence this is var. papilionaceus . The cheilocystidia are really cool.

Zitat von Steve_mt

Yes, I haven't grasped very well the concept of monomitic / dimitic / trimitic, generative hyphae, skeletal hypha, etc when examining under the microscope. They just look like a brush of unspecialised mycelia entangled into each other. I had a look again this morning and I an interesting parasite!

Look what I found in the mycelia of this Inocutis dryophilus. A mycoparasite

Zitat von mollisia

Hello,

the structures shown are not gelatinous, neither the outer layer nor the medullary layer. So Tremiscus is out, and I think all heterobasidiomycetes are out now. They usually have are very loosely interwoven hyphal system with much space for gelatinous matter inbetween.

As the hyphae are clampless, I wouldn't rule out ascomycetes per se. The thin hyphae of the excipulum layer looking like a trichoderm could still belong to an ascomycete.

But another idea: May be it is a very young Cotylidia (pannosa) - I have no experience with this species in immature stadium unluckily.

all the best,

Andreas

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Hi again andreas. I keep your observations in mind.

Not excluding ascomycetes was a surprise for me because all discomycetes I studied had spherical-pyriform-potato-shape packing hyphae in the medulla or sterile parts. Yet I have not observed a large range of ascomycetes under the microscope so as you say, some genera may after all have the tubular-form packing mycelium.

I confirm all you said, the flesh is soft and frial like a sorbet but not gelatinous/slimy/viscous. The hypha were tightly bound and I do not expect they will loosen with maturity. I might recheck again tonight. Maybe I go and buy a couple of red bulls today. I have some five other open posts requiring an update which I wish to tackle today. One of these days I post some extraterrestrial fungi from the past :-p (at least one looked like that and nobody had a clue!) so to be continued...

THANK YOU ALL!

Zitat von ogni volta

Hi Steve, i cannot tell you what this is neither, if T. helvelloides could be an option, ripe fruitbodies should look like this beneath the microscope, you could compare in case. Kind regards, Ingo

Ps: personally I rather not think it is tremiscus. I have no other idea unfortunately. So i am looking forward to how it develops!

Hi Ogni-Volta. You don't have the little man/woman icon lol! I have looked at the link provided showing mycelia of T. helvelloides. One thing in common, there is nothing exciting to see! Your image also fail to show distinct reproductive parts and spores. If I am not tired, I try another round of examinations tonight. I keep you posted and I will travel again to the island I found this population soon.

Zitat von Grüni/Kagi

Zitat von Steve_mt

Ooopsss !!!! I didn't realize you are female - I thought all those who have replied were men. No open intended!

(...)

No problem, Steve! I was just joking, I'm not offended.

Just wanted to let you know who's writing... it's been my fault...you couldn't see it from my new profile picture.

My avatar before this one looked like this:

...by the way, I'm really excited about your mushroom babies and I'm looking forward to the solution.

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Thanks for the artistic explanation Kruni/ Kagi. I never noticed the micro man/woman icon there - so sweet! Nice ex-avatar pic too. I do my best to get a solution. We wait for it to grow a bit more. Lot of nice people here trying to help! I love it. My M.Sc. is really gonna be interesting.

Zitat von Grüni/Kagi

Zitat von Steve_mt

Dear gentlemen, thank you for interest, comments and valuable suggestions about this fungus. I start tackling all your questions here, and micro in the next post. (...)

Hi steve,

dear Lady and Gentlemen would have been nicer ...: clink:

[user = '17652'] Ahemi [/ user]: What a pity that our idea is no more a good idea. I didn't know how they look inside, as I never cut one. : gkopfkratz: Of course you're also right with the color: That of Steve's specimens is far too orange, as one can see in the pic with the cut cuticle.

Ooopsss !!!! I didn't realize you are female - I thought all those who have replied were men. No offence intended!

So my action plan is:

• Open the soil to see if I detect wooden debris that the fruiting bodies are growing from
• Dissect two specimens and dry them 35C
• Leave one specimen to mature at home (if possible)
• Revisit population this Friday maybe specimens are mature and re-examine

A note. The orange inside may mean that the mature basidiocarp will open like a cup having an abaxial (lower surface) the one seeing now and an adaxial (upper) surface, the one inside. On the other hand, the tubular hyphae are distinct for a Basidiomycete, not an ascomycete (usually having spherical / prismatic cells).

Zitat von Oehrling

Hello Herman,

Cortinarius determination by photo is almost never successful, as there are 1000 different cortinaries. There are definitely 100 cortinaries that look like the fungus in your photo.

FG

Oehrling

... and there are 2 or 3 that their micro features are also almost identical too (= sequencing saves the day)

Hi, when taking one specimen, I had pulled it out completely and there was nothing attached to it. So strange, as if it was terrestrial! Here only var. ferrulea is recorded while in the past var. nebrodensis was recorded probably in mistake, and coincidentally for being small and regular in shape. var. ferulae is typically 8 - 25cm, unsymmetrical, eccentric stype, attached to dead old stems of Ferula communis. (huge plant with perennial roots)

Now you must really see this 😱

KR100954964B1 - A novel pleurotus eryngii var. ferulae strain ddl01 and method of producing it - Google Patents

It looks similar isn't it! Colours, shape, small size!

Anyway, i will certainly write back if I have news from Venturella. I write to him today.

Thanks for Yr help. 💗

Images

Zitat von Schupfnudel

This kinda looks Pleurotus to me. I don't know if you guys have Pleurotus eryngii but might be worth checking against. There are several varieties described depending on the host plants so might be worth to check plants nearby too.

Disclaimer: I've never found this species before so I might be wrong.

LG.

Pablo!!! You are correct about Pleurotus . Cylindrical-ellipsoid spores (Q>3), lack of cystidia, pileipellis a cutis of entangled hyphae. Yet I cant believe it is an eryngii if they are 2cm when fully developed, stipe central, no Ferula close by!!! I dry my specimen and show my finding to Prof. Giuseppe Venturella. If its relly eryngii, then it is really variable!

Microscopy:

I dissected the largest specimen I have and clearly it contains a thin cuticle (0.2mm) which is salmon-apricot sitting on a medullary tissue which is white/pale orange. There is a core which is again colorful.

The microscopy is boring and it is obvious that the specimen is immature. No spores has been observed, nor basidia or special reproductive organs. What I have seen is two types of mycelia or hyphal tissue.

1. The cuticle consists of a dense tangled-intricated hyphae about 3um wide and curly / vermicular ending with an acute tip. They have many vacuolues

2. The inner layer consist of parallel running oblong/tubular hyphae, curved/sigmoid but less intertwined or entangled as tissue in 1 and about 8-12 um thick. The transversal hypha are quite straight and perpendicular to the sides. Walls smooth, contents hyaline.

That's it

Dear gentlemen, thank you for interest, comments and valuable suggestions about this fungus. I start tackling all your questions here, and micro in the next post.

After a hard day at work, and sipping down my coffee, i was flipping Zannichelli fungi d'italia and spotted a fungus similar to mine - Guepinia helvelloides (sp. #1545) which is the same as what have been kindly suggested - Tremiscus helvelloides, but then the size (3-12cm) the habitat (mountain) and substrate (wood debris) did not match at all. This is about 1-2cm

Quick answers:

Is it growing on wood in the ground? - I did not see obvious wood parts in the compact calcareous soil, but it was growing under bushes of Pistaccia lentiscus (woody perennial) and it cannot be excluded to have small decaying twigs in the soil - honestly I don't think it is the case.

Dry at 35C for sequencing: Yes I can

Revisit site: It is in a remote place but I can

Infected: No it is healthy

Mature specimens: Those at home no

Is T. helvelloides specific to wood : On the net I see some images of this species surrounded by moss

What does it match : The salmon-apricot colour, the fine pruinosity, the attenuated stipe coloured pale yellow or white, the wrinkled unsmoouth surface.

Microscopy : yes, see post below

I know quite well Pleurotus eryngii which grows on dead Ferula sp. (giant fennel) and I saw a few hundreds of examples and they are pretty large specimens. These were less than 2 cm. I have found this on ground covered by fallen old leaves of Punica granatum. Roots and basal mycelium white with some yellowish tinge and quite deep into the soil (terrestrial ?). There was a distinct root.