Beiträge von Steve_mt

Oh dear me how confusing this !!! To be honest, I did not like much to name a Xerocomus that do not form red dots at the base as Hortiboletus rubellus! And to blow my mind even further, I analysed the sequences in Unite to provide matches as follows:

UDB000443|SH1610853.08FU|Xerocomellus chrysenteron           1585    0.0  UDB000446|SH1610858.08FU|Xerocomellus chrysenteron           1557    0.0  KY693966|SH1610853.08FU|Xerocomellus rubellus                1550    0.0  UDB000445|SH1610856.08FU|Xerocomellus chrysenteron           1544    0.0  UDB000444|SH1610857.08FU|Xerocomellus chrysenteron           1543    0.0  UDB000447|SH1610855.08FU|Xerocomellus chrysenteron           1519    0.0  JQ685725|SH1610853.08FU|Xerocomellus rubellus                1515    0.0  

Now when I click the UDB000443 it says, UNITE taxon name is Xerocomus communis (Bull.) Bon, 1985

... and I am currently running them in PlutoF too (maybe I get different name!)

This is becoming far too complicated!

All 4 samples of this bolete match MN685116

The 2 samples of redeuilhii resulted in:

B408A = ok, 100% Xerocomellus aff. redeuilhii KU721022

B408B = ok, 99.85% Xerocomellus redeuilhii NR_155981

Yes, I expect the hymenium to be in the outer layer. I have not dried 2 specimens so I can do this test but remember that the hymenium is not developed and we might have false negative J test. To be continued .... thanks for your help - much obliged (and applies to all members who have contributed on this coral-lichen thing!)

Neolecta is the closest I could judge.

Neolecta irregularis / Mitrule irrégulière | Récolté par J. … | Flickr

I received the ITS results for two specimens and they are Xerocomellus redeuilhii (one with aff. redeuilhii as if it is a bit atypical, as was my collection

B408A = ok, 100% Xerocomellus aff. redeuilhii KU721022

B408B = ok, 99.85% Xerocomellus redeuilhii NR_155981

https://dergipark.org.tr/tr/download/article-file/899863

The description of H. rubellus in the link below is perfect with what experienced.

Ruby Bolete - Hortiboletus rubellus - Discover the Wild

Dear friends, I carried out ITS sequence for four specimens from this population and had a 100% result for Xerocomus rubellus MN685116 for three and 99.87 for the other.

Now I am wondering at large why non of the specimens produced the characteristic red dots at the base of the stipe and instead it formed a reddening under the cap. I guess I should trust the result but hmmm ?!???

What do you think ?

I can share the sequence results.

I am back with this one and I got the result from ITS sequencing as Psathyrella panaeoloides. As the name suggests this Psathyrella look like a Panaeolus so it is a consolation for me for opting to be a Panaeolus. Schupfnudel you were right on suggesting Psathyrella!!! I was confused between the two but stipe was a bit flexible and the deposits on the cheilos were so much misleading (and the white edged lamellae too)!

Comparing with this excellent report:

Psathyrella-panaeoloidesDaD.pdf

My collection have those ' rectangular ' spores, in the right sizes and some comments make reference to cheilocystidia having deposits on the top. So we are good!

Hi Andreas, Thank you for keeping this in mind and I also have not forgotten this fungs on two aspects.

1. If you need to have a voucher specimen (dry) just to have a look, I can send it. I will sent to Alvarado soon. I suspect it is a new species, I posted on MEditerranean fungi FB and they could not come with good suggestions (somewhat trending on Clavaria).

2. I went to check again the micro of my specimens stored in the fridge and stubbornly no reproduction. The basidiole-like hyphae could indeed be immature asci after all.

So this fungus will go to Spain with my next batch.

Let me know if you need a specimen (Message privately yr address or on info@maltawildplats.com)

I skimmed over the paper - very, very interesting and the pieces are fitting nicely together. This fungus seems quite close to Hypoxylon or Biscogniauxia anamorph, which then becomes a black stroma - the sexual state - on which the Cosmospora grows on (assumingly on the dead stroma, but not sure). Today I will investigate at high power the condiophores. Biscogniauxia forms black stroma on bark so we are getting close!

Well done guys - I come back in the next competition

My favourite one was the Lepista which came 2nd for its subtle colors, angle and soft focus in the border.

Thank you xxxx

I will investigate and let you know. To tell you the whole story, I think when its life is over it deposits as a black layer on the bark, on which an interesting Cosmospora s.l. sp. (cf flavoviridis) later grows upon. I really wish to solve this puzzle so many thanks for your guidance.

Nectria cinnabarina s.l. (or something else) - Forum ASCOFrance

Hi Andreas, thanks for yr help. I have sent material to Alvarado and I can send this to him too. However if u wish to have a sample to examine under yr lens, I can send you one fruiting body. When they dry they are quite small and shrivelled so hope they rehydrate well. Let me know (send address). The samples in the fridge are also healthy and will check their maturity tmrw

Photos in situ....

Hi, here I am writing again about another strange fungus growing on the bark of a dead tree that I suspect is Ficus carica. First seen, it looks like a dry coral mushroom with a pale beige-ink color. It grows in tufts and then it apparently blackens out. Under the microscope, it turned out to be a synematous microfungus composed of sterile structural core of thick entangled hyphae, mustard-brown to curry-brown in color but dull. This becomes black in KOH. The outer layer is a thick dense coat of penicillate hyphae, with branches arranged in whorls and each subbranch seems to have a small head of phialides each producing one conidiospore. Conidiosore bean-shaped. This layer is pale mauve (almost greyish) and turns dirty green in KOH. The conidiospores are cream-straw color and become mauve-pink in KOH.

any clues please?

Thanks, I try to stay away from FB coz I waste a lot of time, but I think everyone is running out of ideas, and maybe I should post there. However, while the fungus remains in a sterile condition, we can't expect miracles. And I don't feel like going again to the island of Comino for the third time !!

Zitat von KaMaMa

Hello Steve,

Water will evaporate out, diffusion is just a matter of time ...

But anyway you can't propably open the dish slowly enough, without creating turbulances, transporting spores inside.

Surely won't work. Sorry, silly idea of me.

Best regards, Martin

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It's Ok Martin don't worry, we share opinions without any regret! I was also a bit silly to think it may take 3-4 days to evaporate, but it was quicker (at 40C). Thanks for your thoughts!

I managed to dry it with the filter paper method after sterelizing it in the microwave at 440W for 5 minutes. Maybe I am making lot of fuss for nothing because the agar dried in c. 30hrs and that is too short time for any serious contamination.

Now what about storage? I have in mind to l cut the agar around the colony wrap it in aluminium foil and put it in close dry environment, perhaps tupperware container with some silica gel. I am sure insects will feast on the agar if I leave it in a box. I have lot of silverfish in my house recently (probably they are attracted to fingi)

Zitat von KaMaMa

Hi steve,

Firts of all, I haven't done this before, but why don't you turn yout dish upside-down, remove the cover and dry it like this?

Contaminating spores won't move upwards, I think.

Regards, Martin

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That way the water can't evaporate out

Under the microscope, there is more or less the same picture of before, but what we assume that are basidioles are more wriggly (curved/flexuose).

Hello... Sorry for letting you wait.

Here are some close-up images of the fruiting bodies, 6 days after the first encounter. The have not changed much. They seem to have more a coral-fungus approach then that of a jelly baby fungus. The flesh is soft and breaks away easily, but decivesly not gelatinous. I have dried some more specimens and placed few in the fridge. Not the heavy pruinosity near the stipe.

Found them. 3 populations. Here are quick mobile photos 😍

I take some pics with slr. They did not change that much. Cu

We are going for it. Hope we find it in goid shape 🙏😊

You hit the nail on its head re last fungus. I see if I can do more re species level! Interesting!

Thanks!

Ceratellopsis

But spores, bacteria and living matter that have water inside so the microwave radiation "should" destroy them, at least theoretically. The question is if the rating of 440W is enough, because higher ratings damage plastic, wood/paper, rubber and arcs with metals etc.

Microwave sterilization - PubMed

Effect of microwave radiation on Bacillus subtilis spores - PubMed

Yet these claims do not mention the power rating So I wonder if someone had personal experience.

Wish to have confirmation on my ID of Mycena hiemalis for this wood-inhabiting mycena. Saprotrophic on Ceratonia siliqua

I know that microwave should kill all living matter. When it comes to plastic or paper or metals, setting at high-power can cause burns and damages to such materials, but at 270W and 330 W (I think also 440W) no damage is created. Do you know if at these ratings for long period (say 10mins) is an effective way of killing bacteria and fungi spores. Sometimes I re-use petridishes coz they are expensive.