Hi, I often see questions on FB and online fora, asking how to get ascospores out of asci for proper measurement. I have this easy method to share with you which also works on tiny apothecia, even if dirty with soil or debris.
A droplet of water or cotton blue is placed on an upright cupulate or discoid apothecium with a fine-tip dropper. The water is agitated with a needle gently touching the surface. The spores are in suspension of this droplet. This is then transferred on a microscope glass slide and mounted for observation. If the apothecium was dirty with soil, let 10 seconds or more after agitation so that the heavy soil falls down and not sucked by the pipette.
How someone finds it useful