Beiträge von Steve_mt

Thank you Julia for your time and for sharing your expertise

All the best

Stephen

Dear friends.

We have a 100% confirmation from DNA sequencing and genetic matching thanks to the assistance of Uwe Winkler and Dimar Balint. It corresponds to Cortinarius ayanamii A. Ortega, Vila, Bidaud & Llimona

Thank you Julia, investigation solved then. 😎. I have two further questions

1. Am I right to assume that it won't kill the host plant? Infection seems to be effecting only sone florets (5-20%)

2. Is the fungus 'dormant' in the host perennially and then emerges with the inflorescence.

Thank you,

In the meantime I had a look at the spores, and they dont seem particularly interesting although i have a curiosity. The spores are globular with a preipheral rim (thick wall?) and amorphous semi-translucent bodies which I think they are guttulae. The spherical spores had a diameter of 5.0-5.8 um (see details below). Surface smooth sometimes with some eroded portions in the wall. The images are froum spores mounted in an anti-surfactant and KOH (3%).

The curious part is that within the spores mass there were spinulose spores which I judged as doubtfully artifacts/foreign extrinsic matter, but they were definitely in the spore mass extracted from the glume pockets. They were rare (1-2 in 200 spores in one slide, 1-2 in 80 spores in another). They measure about 12um. I thought it is worth mentioning.

Spore measurements

5.28 5.28

4.98 4.72

5.64 4.44

5.24 5.11

5.53 4.68

5.05 4.72

5.02 4.87

4.57 4.42

5.99 5.31

4.76 4.55

5.11 4.79

5.02 5.02

5.19 5.19

5.47 5.10

5.68 5.15

5.99 5.39

5.62 5.09

5.00 4.77

5.19 4.47

4.17 4.05

5.78 4.95

5.57 5.04

5.16 4.90

5.41 5.29

4.97 4.73

5.52 4.94

5.25 5.13

5.13 5.02

6.26 5.55

(4.2) 5 - 5.8 (6.3) × (4.1) 4.5 - 5.3 (5.6) µm

Q = 1 - 1.2 (1.3) ; N = 29

Me = 5.3 × 4.9 µm ; Qe = 1.1

Thank you Stefan for your message and moving the post. I follow this fungal pathogen as much as possible.

Is Julia on this forum or I have to email her?

Thanks

Hi, years ago I found dark rust fungal spore mass inside the inflorescences of Lygeum spartium (Esperanto grass). Some five years later I still see the same infection in the same population. I am curious to investigate. For the moment, I can share only macro features and will hopefully work on the micro tomorrow and try to cultivate on culture media next week.

The dark brown spores only exhibits themselves inside the single floret of the species. Apart from its presence, it also deforms the flowers making it look to have 2-4 florets but it may be that each corresponds to the two glumes, lemma and palea (infection is causing dysplasia). One member (Bjorne) suggested and sp. which is probably correct, but a further resolution to species level is desired

It should be some species of Penicillium.

I revisited the same population and just collected a few infected inflorescences. I post on a fresh topic in the forum. Bjorne, if you want to follow just look in the respective topic-group where I should post this again. I will have a look at the spores tomorrow

https://www.123pilzsuche.de/daten/details/Weisse-Adern-Dachpilz.htm

Pluteus inquilinus - Funghi Non Commestibili o Sospetti - Funghi in Italia - Fiori in Italia - Forum Micologia e Botanica

Inquilinus seems to be a more petite fungus with semi-translucent stipe, and a whiter -> whitish-cream pileus ... but closely related of course. I am just confused/undecided between inquilinus and plautus.

Further comments are welcomed!

I am convinced that it is a washed-out melantina. Thanks a lot for your replies!

Dear friends, sorry for the late reply. I was inquiring and researching the possibilities based on your kind answers. I have found a closely related species of P. inquilinus which often grows on oak forests, hence a pale form of P. plautus. Could this species be considered? I know usually it is medium brown in color, but paler forms are often documented and met in the wild:

Whitish roof mushroom, different colored roof mushroom, velvet-footed roof mushroom, bulbous roof mushroom (PLUTEUS PLAUTUS SYN.PLUTEUS DEPAUPERATUS)

spore:(6.5) 6.8-7.4 (-7.8) x (4.8)5.1-5.9(-7.8)

The link above shows strong similarities!

Pluteus plautus (Weinm.) Gillet 1876 - Funghi Non Commestibili o Sospetti - Funghi in Italia - Fiori in Italia - Forum Micologia e Botanica

Spore 6.6-7.9 x 5.9-7.2 µm; media 7.3 x 6.6 µm; Q = 1.0-1.2; M = 1.1, as a subglobose largamente ellissoidali, lisce, con apicolo, guttulate

This is also valid for terrestrial orchids! Very nice quote from the mycologist.

Zitat von Peter

Hi,

nice that you speak English so well.

There was once the saying of an experienced mycologist (who was also familiar with the publication by HILBER): "Everyone who describes new species should be forced to spend 1 year in an oyster mushroom cultivation facility beforehand. Then not every small change is recorded as a new species. "

regards

Peter

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If you want this sample and other samples from Malta and sometimes Finland - I will help

Not being any mushrooms around, I am trying to re-investigate old collections. I have found this collection in an oak forest (Quercus ilex) which is beige, medium-small (8cm long, pileus 4.5 cm wide). It had a fragile stipe but I don't think it is a Psathyrellaceae for its spores are subglobular and hyaline giving a light cinnamon-brown spore print (but I am aware of how diverse this family is!!!). Gills free. Cheilos present. No ornamentation on the cap, no smell, no volva, no ring, no bruising, no reaction, very boring! Basidia clavate, x4-sporous. Frequent, variable in size, utriform (45-90um long), pleurocystidia rare or absent. Spores 5.1-7.3 x 4.1x5.9 µm, subglobular-pip shaped. 'Hymenoderme of subglobular to broadly oval, slightly angular hyphae with a very short appendiculate peduncle. If I have to guess, I chose the genus pluteus. I don't have an exsiccatum for further investigations

Hi dear friends,

I. wanted to chare this beautiful species and confirm if it is Helvella leucomelanea.

Well done Claudia and many thanks for this forum members and respondents - I am learning a lot!

Oh well, yes for the fact that P./C. melantina is very common in Mslta and grows in similar habitat and habit (with spores matching). I forgot that this particular species have pale spores. I did rehydrate the old specimens in 5%KOH before staining and then cleaning in glycerol/KOH mixture. it is a good suggestion, thanks! I will check more images of this species as probably when photographed in situ they were overmature, wet, and weathering (eroding) away. thanks as always !

Section in Lugol's iodine - spores inamyloid

The spores are ovoid, hyaline, smooth, with an eccentric tiny apiculum. Guttulae not seen and some are kinda broken, maybe because they are old spores(?). I can see variability in size too (9-12um long). If necessary I can test if they are amyloid.

(8.8) 9.2 - 11.4 (12.1) × (4) 5.2 - 6.4 (7.1) µm

Q = (1.5) 1.6-1.9 (2.2); N = 17

Me = 10 x 5.6 µm; Qe = 1.8

Here is the section mount of another gill. The edge does not show cheilocystidia unless they got eroded or destroyed during drying / preservation. Interestingly, there are what looks as subglobular cystidia in the gill face, perhaps corresponding to pleurocystidia (?), The largest being 35x 25 um.

The microscopy is pretty disappointing. Pleuro absent, cheilo probably also, basidia not distinct and spores are quite translucent, hence I am thinking that this is not a Psathyrella. I try another attempt and I will also measure the spores.

Dried ex-siccatum found.

First hint - lamellae become translucid (colourless) when hydrated in KOH. Spore mass colour (spore print) seems to be tobacco brown - just an indication. Now trying to hunt for spores and cheilocystidia

Lot of basidioles but seldom defined basidia sterigmata. The few I saw where x 2 (-3). What do you think, do you agree from these photos ?

I have another fungus that I am trying to have a clue. I made up my mind that it is a Psathyrellaceae. Interesting is the zonation on the pileus and the its very viscid character. It was growing from a pile of old stems of dumped plants. Maybe wild plants from a nearby field. I see if I have collection for a micro assessment. Any early clues are well appreciated. Thanks!