Beiträge von Steve_mt

Thanks bjorne - I had my browser set to - automatic translate to english and the chapters of the book were showing in English on Springer website. Pity the book is in German. Wish it will get translated soon or later as it seems to be a great reference and unique book (maybe if I buy the pdf where I can copy text and get the text block translated in Google translate.... but not sure if the pdf let u to copy text to be pasted (sometimes they don't allow it for copyright)

thanks

Hello Bjorn! Thanks for the detailed reply and it was very helpful.

Starting from the last, the book should be this one:

Pflanzenparasitische Kleinpilze | SpringerLink

Maybe I buy the e-book at half the price.

Regards our rust, I scraped immediately my idea of P. striiformis and it is most likely to be Uromyces lineolatus. From the aspect of host ecology (life cycle) there are several Apiaceae in the surroundings (Daucus, Ferula and Phoeniculum) while there are no Nymphoides recorded in Malta (and cultivated chances has to be discarded as the locality is away from urban). I can mount some spores in glycerol solution and see if they germinate to have the 100% mark.

The 1959 book is here (german text )

https://swisscovery.slsp.ch/permalink/41SLSP_NETWORK/nan79r/cdi_eth_eperiodica_oai_agora_ch_cry_001_1959_12_1460

THANKS

Uromyces lineolatus | (Obligat) Phytoparasitische Kleinpilze

Uromyces lineolatus ?

"this rust fungus performs a host change. While the spermogonia and aecia are formed on umbelliferae (Apiaceae), the fungus switches to sea rush ( Bolboschoenus ) in summer for the formation of uredia and telia ."

This is a Pucciniaforming pockets elongated from where the spores emerge and released. I think Identification of Puccinia is somewhat host-related. I don't know if there is a website/database where one can input the host and get a set of possible plant pathogens.

Spores

(23.6) 24.7 - 32.1 (32.5) × (16.4) 17.2 - 19.8 (20.1) µm

Q = (1.2) 1.25 - 1.8 (1.9) ; N=9

V = (4018) 4518 - 5293 (5427) µm3

Me = 27.1 × 18.6 µm ; Qe = 1.5 ; Ve = 4892 µm3

and spore wall is almost 2 um thick!

Well if you have some information to share with me about this species I will be glad.

pilzforum.eu/attachment/426295/pilzforum.eu/attachment/426296/

Hello guys... I am really busy concluding my thesis (on macromycetes) of which deadline is in a few weeks now. I think many remember this post and its mysterious orange coral-like fungus, where I sent samples for DNA analysis. Few days ago I received the DNA ITS1 results and disappointingly Dr Alvarado told me it was noisy or contaminated and suggested to repeat. I have the fasta file (I can share with whome is interested to compare) but maybe its wise to wait the repeat. I am quite sure I did not contaminate the specimens.

Thanks for replying Thorben. Ok we exclude Trichoderma. When I am not busy I try to match it graphically or try to key it out. I posted it because it is quite beautiful.

I wonder if this is another Trichoderma sp., Different from the one I recently posted.

This is also a contaminant while trying to isolate Cosmospora from organic substrate on culture media

Tirchoderma is a good option here or there are other genera please ? The perpendicular profuse branching and fluffy-green colonies do match.

Thanks, I selected this mold for sequencing - I keep u posted - hope it works out.

1. Dried agar at 35-39 C

2. Cut agar into small pieces (quarters)

3. Placed in an aluminum pocket

4. Then in a paper pouch

5. Placed paper pouched in a bag with silica gel

P..s. in the agar I added antibiotics to prevent bacterial growths (it helps a lot) hope this would not interfere with the sequencing!

Thanks, yes. Not very human-friendly pet!

Thanks

Some more data

Colonies had a strangeoff-putting rubbery scent (I think I should not have smelled it!)

On applying KOH the spores turns dull brown

The conidiophore os smooth, thin

The heads are two-series, the metulae elongated trapezoid, the phialides short

Metulae 20-28 um long

Phialides 7-8 um long

Spores 3-4 um across, smooth

According to my simple book (but I love its keys) this would be A. flavus (easy peasy as that ? I don't think so !) but I am seeing colonies of A. parasiticus offer a closer match!

Here are some microscopical data.

The heads are in my opinion une-seriate, but better confirm from the images. The spores are spinulose, 5-6 um, they do not change colour in KOH. The conidiophores are thick and widen close to the head and there is some 'wrinkling ridges' just below the head (characteristic ???) and covered with dense small warts. Phialides are about 12-15 um long with tapering tips. Conidiospores are produced in long chains and are difficult to separate from the heads. The heads are quite strong, I could not break one to get loose the phialides!

Zitat von Florian1986

Hi Steve,

i am afraid that species determination in this genus is not possible without molecular data. Currently there are more than 450 species in this genus and i am sure, that there are still a lot of species, which are undescribed yet.

Best regards,

Florian

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Yeah I was afraid from getting this reply. Actually, I am drying some agars maybe I sent them to Pablo.

OK, I will do that right now. Microfungi always astonish me under the microscope but they are often disappointing re identification esp Aspergillus and gulp! Penicillium! I don't have DG18 I see from where I can buy it (do you know some good online shop that the prices are for home mycologists !

Hello, some time was dedicated to this astonishing fungus. First of all, this was growing on the base of the trunk of a complete burnt Ceratonia siliqua tree, in the sense that I think it was dead. Other burnt carob trees in the vicinity had new branches about 1m long sprouting after the September-November rain.

Now I examined the specimen which assumed an ochre-buff colour for being completely covered in spores. It appears that the fungus consists of repeating indeterminate budding, each bud about 4mm across. Beneath there is a gelatinous matrix with an olive-green colour that does not change in alkaline solution.

As expected, the reproductive part is carpeting the outer layer and could judge that they are conidiophores, like cactus trees with stout meticulae (or phialides) where at the apex there is a head of tiny bristle-like appendages where (conidia-)spores were attached to, hence looking like a Nodulosporum! Some hyphae had walls with incrusted pigment.

So now I am convinced that this is an Ascomycete, an anamorph of Xylariaceae, possibly a Hypoxylon? The fungus looks to be a Nodulosporum or look-alikes.

Another Aspergillus,growing on dead succulent stems of Aptenia - this time coal black colour . Aspergillus nigra could be one, are there other black aspergilli spp. pls. ?

Sorry for the jumbled sequence - here are the agars in sequence of position in the abovepost

1. PDA

2. Czapek

3. Malt Extract

4. Malt Extract

5. Czapek

I attach here reverse plate of PDA

I have collected this Aspergillus from the fleshy mesocarp (and exocarp) of Iris seeds. I cultivated it and got this beautiful green colony with chlorine-yellow green hues. A quick look under the microscope and it was an Aspergillus sp. I had dense conidiophores under the microscope and nothing photogenic, but I try the scotch tape method tomorrow. I wonder if there is a specific aspergillus which gives the colour combination.

It was less colourful on Malt Ext Agar and very colourful on Czapek. I am cultivating it on Oat Meal Agar too just to check how it looks.

Hello, I was doing some culturing experiments / research, so I will be posting a couple of posts in this section. Hope there are colleagues who are interested.

I.am trying to subculture pleurotus spp on media. Sometimes they are slightly parasitised or maybe plate gets contaminated. I found this beautiful 'broccoli-colonies' mould (for their scattered green small heads) and under the microscope there are small ovoid-elliptic spores, perpendicular branching and single ellipsoid-fusiform phialides giving rise to what I think cluster of spores.

I think we are dealing with a Trichoderma. What do you think ?

Thanks Jan-Arne,

I think you hit the nail on the head. Looks a good comparison

Hohenbuehelia cyphelliformis (Hlívička číšovcovitá)

Hohenbuehelia cyphelliformis - Becherförmiger Muscheling

Another option is H. grisea but that species is more grey

In my archives I have this small fan-shaped bracket fungi, about 2 cm across, growing on fallen branches of Ficus carica (Fig tree). The hymenium is waxy white, very spaced up and turning beige. I was thinking about Panellus (serotinus maybe) but could be other fleshy species. Help is appreciated

Probably you are referring to S. incrustans

MycoDB : Fiche de Sebacina incrustans

I halted my investigation but when it dries it become like a coral body covered in ocre spore powder as in the pics of the link above. However that species is a grass species but this was on a burnt trunk.

Many thanks,,, to be continued

I will investigate. Quick response: This is the anamorph of Hypoxylon deusta which looks like a black amorphous stroma and I could see something like that on the trunk (I thought it was soot from the burning of this tree!)