I am thinking that we should be around Cystolepiota for that fringe and flocculose cap.
Beiträge von Steve_mt
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Hello, I am back on track to identify this small mushroom. Gills are free (or slightly adnexed). Stipe bruises a little (?); cap floccose and appendiculate
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Hi, can someone help me to find this document:
Synoptic Keys to the Sclerotiniaceae & Rutstroemiaceae in Nordic Countries [Norway, English]
Look here, Steve.
0 Synoptic keys to the inoperculate stromatic discomycetes in the Nordic countries.pdf
But if it will help you?
If you're interested in a special genus, ask me.
There are some more documents from Schumacher & Holst-Jensen, which I can send.
Regards, Nobi
I am interested in the genus Sclerotinia - I only found old literature regarding morpghological identification of three related species. I believe my specimen is S. sclerotiorium
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Good afternoon dear friends! I wish to thank you for your help. I finished my writeup about this species and I agree that all species in the genus have a Sclerotium and turns out that it is very diagnostic to distinguish the species. I had to base my research on old literature:
- Hall, R. & Boland, G.J. (1994). Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology 16:93–108. doi:10.1080/07060669409500766
- Willets, H.J. & Wong, J. A.-L. (1980). The Biology of Sclerotina sclerotiorum, S. trifoliorum and S. minor with emphasis on specific nomenclature. The Botanical Review 46(2):101–165
- Kohn, L.M. (1979). A monographic revision of the genus Sclerotinia. Mycotaxon 9: 365–444
Three related species are S. trifoliorium and S. minor but my specimen corresponds to S. sclerotiorium . Interestingly it was associated to Chlorophyton comosum, the only herbaceous weed that was present on site, and then a tree of Bay Laurel.
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Hi, can someone help me to find this document:
Synoptic Keys to the Sclerotiniaceae & Rutstroemiaceae in Nordic Countries [Norway, English]
The link for the file is broken on that site.
I.s the black sclerotium typical/distinctive for the species? (seeing its epithet is sclerotiorium)
Thanks
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Thank u Stefan for confirming 🤗
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Do you confirm Coprinellus radians from the spore shape and size (and general morphology) ove xanthothrix?
7.8 [9.1; 9.8] 11 × 5.2 [5.9; 6.3] 6.9 µm
Q = 1.3 [1.5; 1.6] 1.8; N = 21; C = 95%
Me = 9.4 x 6.1 µm; Qe = 1.6
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Can you help me on this one ? I have a dejavu I already posted it somewhere but I was off d=from fungi for 3 weeks due to health. The mushroom is small, (2cm long) completely white. Spores ovate, appiculate, tiny and about 4um long, hyaline and do not stain well. Clamp junctions observed in the pileipellis.
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Dear Cristoph,
I don't know you but your story touched me, both regarding your stress/overload (I'm in a similar position) and that of your mother. I hope you can find the strength and morale to fight a bit more and get out of the dark tunnel - friends and fungi are awaiting you in the sunlight.
All the best
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So this is clearly flava because the lower layer of lime in the 'crust' is yellow? That what is meant by inner lime right?
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I have found this slime mold high on a trunk of a Tamarix africana about 1m above ground level (strange place for slime moudds) and I am quite sure it is Fuligo septica sl but then I am not sure about the variety. It has a pale yellow / cream outer lime surface followed by a bright lemon-yellow one below. The capillary looks white. I am undecided between var. Septica or var. Flava?
y a
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Dear friends. I think i found the problem. It was the oil. I bought oil for microscopy (professional) and i am getting the correct x1000 magnification (x980 to be precise) everytime. I will only buy when i see the label of the reagent from now on!
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I try to record on video what i am doing, but how can i share on this forum? It only allows jpgs etc not vids (mp4). I am also very concerned, also for my msc. Methodologuy
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To prepare 10% ammonium hydroxide solution, should 1 + 9 parts: Conc NH4OH solution & water be mixed or about 1 + 3 parts since conc. ammonia solution is 25-30% concentrated (not 100%). ?
Thanks (i believe the second one! ;-))
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Hi everybody,
@Steve, good news!
But you still have a problem with your optics, probably the condensor is not adjusted. The lines of the eyepiece reticle have a blue shade towards the center, do you see that? And the lines of the reticle are still distorted to the corner, maybe you placed it the wrong way round (resulting in a wrong optical layer for the reticle).
If you spend so much money for buying the best tools, you should also spend some more time to learn better how to use them.
Also you should also spend a few Euro for oil with a guaranteed optimum refractory index, this might improve the results further.
The newer Zeiss and Olympus lenses should have a correction factor very close to 1,000.
... and sell the useless 63x dry and buy a 40x oil instead
Wolfgang
I think the problem is the camera method because I put my Canon G5X over the eyepiece and zoom a bit to fill the field. When I look with my eyes, I do not see the pincushion distortion. For the colour aberration, I see them but maybe I am not very touchy here, but in real life situation, they are not really significant or a big deal at that high magnification. Sometimes I turn the almost moncromatic photos to greyscale.
Saying that, I do not know much about use of the condenser. I usually raise it until I have good contast. Too high I lose the contrast and result in getting a soft image, too down then I have too high contrast and blackened image. Usually, I test with my eye until I have rather good contrast and the blur washes away the background artefacts and dirt. I admit my setup is below average too
- but for now that what I can afford. I miss a trinocular. -
Hello Uwe
yes, that's exactly how I do it. Oil drops directly on the stage micrometer. The idea of putting a drop of liquid and a cover slip on top of it has never even occurred to me. The literature available to me says nothing about this.
best regards,
Andreas
Hmmm.... for me that was the problem, so double check or can you confirm for us that u have no magnification error like what I reported ?
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[...]
... and sell the useless 63x dry and buy a 40x oil instead
[...]
PNF 40x/1,3 Oil: I love it!
Like this ?
Yes not very good optics the 630, I use it mostly for measurements (gives a bit more accurate results). I am not very fond of messing with oil, but maybe I should change mentality...
Do you mean like this?
Carl Zeiss Microscopy, LLC - Objective Assistant - Objective C Plan-Apochromat 40x/1.3 Oil DIC M27
OMG!!! so expensive!!!!
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Dear Andreas, unfortunately, I could not photograph the poorly stained spores very well, but they had one septum. an elongated fusiform shape and usually 4 oil drops. I can confirm that the asci were inamyloid at the tips. Another thing, the ascocarps were growing on bark of a dead olive branch and associated (or on) pyrenomycetes over the bark. All these are indicators for C. sulfurina from the small amount of research I did.
I used Lugol's Iodine solution - I don't know... but it works for me to stain the Asci tips and less dangerous. I do have Melzer's, so if crucial I can test the asci with it again.
I am very disappointed with my image qualities of thid fungus - sometimes I get good images and sometimes I don't. This did not stain very well. I photograph stuff from my Digital camera through the eyepiece. (my microscope is not trinocular
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Flickriver: Searching for photos matching 'Bisporella sulfurina'
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Is "Calycina sulphurea" synonym to "Bisporella claroflava"?LG; Pablo.
Hello Pablo,
yepp.
I worte incorrect "sulphurea" but I meant "sulfurina". Bisporella sulfurina = Calycina sulfurina = Calycina claroflava
all the best,
Andreas
I am lost in nomenclature : Bisporella sulfurina = Calycina sulfurina = Calycina claroflava = Calycella sulfurina ?
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Hi Andreas
He may have put the oil directly on the micrometer object without a cover slip.
Greeting
Uwe
THAT WHAT I USED TO DO!!! when I get the 86 units per 100um (the wrong magnification!)
Now I placed a coverslip over the micrometer fixed coverslip and oil over the second coverslip and I got 98:100. This was so sweaty to arrive to a solution of this mysetrious error. So now my calibrations a slightly screwed when I measured under the x100 but at least not by much (12% error).
I cant understand the physics why a coverslip makes this difference ?!?!
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OMG!!!! It worked!!!!
I have 97-98 reticle units for 100 um!
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I can try micrometer+coverslip over+oil >>> objective and see what I get, but I think I am going to have > 0.17mm working distance
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the x100 (no oil) x 1.05um per reticule decision (calculated magnification x 960)
the x100 (with oil) x 1.17um per reticule decision (calculated magnification x 850)
How have you done this measurement ?
1: test without oil : have you used a cover glas and water inbetween the measurement slide and the objective ?
2: test with oil: have you used a cover glas and water inbetween the measuement slide and the objective ?
This objectives are calculated for 0,17mm coverglas usageBR
Uwe
without oil: Micrometer slide on stage, then no water no cover glass slip (direct visualization)
with oil: Micrometer slide on stage, then droplet of oil on micrometer no cover glass (objective lens in oil)
The micrometer already have a fixed coverslip I don't see why I have to put another one over it and water.
Alles anzeigenthe x100 (no oil) x 1.05um per reticule decision (calculated magnification x 960)
the x100 (with oil) x 1.17um per reticule decision (calculated magnification x 850)
How have you done this measurement ?
1: test without oil : have you used a cover glas and water inbetween the measurement slide and the objective ?
2: test with oil: have you used a cover glas and water inbetween the measuement slide and the objective ?
This objectives are calculated for 0,17mm coverglas usageBR
Uwe
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pilzforum.eu/attachment/363503/I have carried out the micro of the yellow asco. From some observations under the stereo, first impressions are that the pale ones are the same one more or less decolorized because some of them have a bit of yellow pigment in them. The atypical border is perhaps because they are dry ?!
The asci are dextrinoid or stain strongly in IKI except where there are the spores , otherwise inamyloid at the tips; 60-80um long and only 5 um wide. The elongated spores overlap each other in the ascus. The spores are interesting and maybe do not corresponding with C. citrina. They are narrowly fusiform, almost linear with four bands due to the presence of four evenly spaced guttulae. They measuring roughly 10-14 µm x 2-3 µm. They do not stain well in Congo red or IKI. I believe there is a central septum.
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Let me write about the procedure, just in case... I mount the stage micrometer which has a very thin and small circular coverslip fixed to the glass slide, transfer a small drop of oil over its surface, mount, and bring to focus the scale. Then I place the reticule eyepiece (I have 3 of them) and align it so the zero marking is over the end of the stage marking. I count how many reticule markings make a known measurement and calibrate accordingly
the x10 = x10um per reticule division (calculated magnification x 100)
the x40 = x 2.51um per reticule division (calculated magnification x 398)
the x63 = x 1.59um per reticule division (calculated magnification x 628)
the x100 (no oil) x 1.05um per reticule division (calculated magnification x 960)
the x100 (with oil) x 1.17um per reticule division (calculated magnification x 850)
