Beiträge von Steve_mt

I just discovered this cute tiny fungus (not really mould or microfungus so hope here is a good place to post) and wish to share with you. It was growing on several pinnae (leaves) of Phoenix cf dactylifera.

Now I want to identify this Aspergillus which was growing on the Parsley flowers and fruit together with the Erysiphe

Part 2: How did I manage to isolate the Aspergillus!

I took a the plate which had a dominant colonization of aspergillus, remove the lid, and tapped gently over a plate of innoculated Oat Agar. Theoretically, only spores of Aspergillus should drop.

Result: Success (plus a tiny contaminant of A. niger! - very persistent )

-)

So here we have a situation of buy one and get one free. I cultured the microfungus by touching the infected fruits on petri dishes, and what we have: two microfungi - The Erysiphe with many white unornamented Chasmotheca intermixed with an Aspergillus species forming beautiful cyan / sky blue conidiophores.

I tried to isolate the two by careful inoculation by I got them both growing every time

Agree, I just find it strange the growth is only manifested on the inflorescence!

Thanks for replying

Here is a link re disease of parsley (general info)

http://ausvegvic.com.au/pdf/Parsley_Disease_Handbook_2006.pdf

So I am still working on this Penicillium sp., extracted from the cartoon of wine which on Oat meal agar and especially on Malt Extract Agar, the colonies form a deep, bright red exudate. I have examined the penicillate conidiophores which are biverticillate and rather slender and it is matching with P. pupurogenum is close enough. Still, maybe there are similar species to consider.

Is it normal for this species

to grow only on the fruit?

Thanks, I check the prices, and if they deliver to M Alta coz These are the top Lab suppliers and I do not need Grade A stuff...

Thanks mate

Is there a particular species of Erysiphe that grows specifically on the fruit and flowers of Petroselinum crispum (not on the leaves). The leaves are intact. Logic says E. heraclei but not so sure since of its specificity on the reproductive parts of the plant.

Zitat von Cyathus

https://www.myko-service.de/p/flora-agaricina-neerlandica-vol-7-noordeloos-me-et-al-boletales-lactarius

best regards

Axel

I am trying to order it. 80Eur is worthed (NHBS is ca. 150 Eur!)

See if ths helps

Zitat von Cortinarius

Hi Steve

You can use different Genus, which are in general mycorrhizal

ground floor Cortinarius, Inocybe, Hebeoma, Russula, Lactarius, Amanita, most of Boletales

...

As I know for aforestation

Leveroma is a good choice

BR

Uwe

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Thanks UWE, is it as simple as that ??

Many thanks for all your help, and sorry for my late reply.

You are suggesting a number of genera, but how do you know that they are (from experience and articles probably) but I would love to buy a book (or paper) listing numerous examples and which trees they are suitable for. From experience, I know Suillus collinitus is mycorrhizal with Pinus... but its would be great if I can have some reference book.

E.g.

Mycorrhizal Fungi in South America (2019)- Pagano, Marcela C., Lugo, Mónica A. (Eds.)

Mycorrhizal Fungi in South America (2019)- Pagano, Marcela C., Lugo, Mónica A. (Eds.) - Details the ecology of different types and species of mycorrhizas…

www.mykoweb.eu

(...but for Europe?)

Hi, I wonder if someone has the whole collection of these wonderful monographs. I miss volume 7 (pdf)

:coffee:

ITS Results suggested Aspergillus pseudoglaucus and comparing quickly the morphology it should be ok. The second option is C. cibarius.

Polyphasic taxonomy of Aspergillus section Aspergillus (formerly Eurotium), and its occurrence in indoor environments and food

Aspergillus section Aspergillus (formerly the genus Eurotium) includes xerophilic species with uniseriate conidiophores, globose to subglobose vesicle…

www.sciencedirect.com

I have results from ITS sequencing which is suggesting:

1. Ampelomyces sp. (HQ649997)

2. Stagonosporopsis cucurbitacearum (MT312750)

Must be the second one for the presence of Pycnidia

Dear friends,

Today I received several results of ITS sequencing of moulds. This post is specifically about a Cladosporium where the same colony was subculture into two different plates (U11a and U11b), and I submitted both for sequencing. The following results are received (I have fasta / ab1 files for both):

U11a = ok, 100% Cladosporium tenuissimum MK513837, C. cladosporioides MF475952, Hanseniaspora uvarum MG250496

U11b = ok, 100% Cladosporium cladosporioides MT466517,C.cubuliforme MT312738

So I have a tie (undecided) between C.cladosporioides and Cladosporium tenuissimum.

How can I decide which is the one???

1. Morphological examination (can it tell the difference between the two) ?

2. Do other molecular tests (LSU ...???)

3. Edit the sequences and repeat the phylogeny on BLAST/MEga-X

4. Plate on different media?

I prefer the cheaper options (hence avoid doing more tests! unless there are other cheaper options!)

I am an eager amateur

Zitat von thorben96

Hello Hagen,

that doesn't appear to be Penicillium, but you don't show how the conidia are formed.

Because it is important to see whether the conidia are formed in chains or perhaps only individually, but the shape of the conidiophores is also important.

I suspect the conidia are pinched off in long chains.

VG: Thorben

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Fully agree, forget it is an Aspergaceae

After reading some literature, Talaromyces purpurogenus is my best guess. It is used as food colouring. Another clue is that it did not forned red pigments on PDA and I saw that this is so for this species

Safety Evaluation of Fungal Pigments for Food Applications

Pigments play a major role in many industries. Natural colors are usually much safer when compared to synthetic colors and may even possess some medicinal…

www.mdpi.com

My hypothesis is that Penicillium purpurogenum pigment was used in this cooking wine, and some spores might have escaped in the wine. (BTW cheap cooking wine). It used the soaked carton box as a, substrate to grow.

Amazing if this is the case.

When I'm back home from hospital, I carry out some microscopical investigations.

Thanks, but the task is a bit different, I have to mark from a list of 300 macrofungi which are mycorrhizal to be implemented in aforestation etc.

Hi, after my M.Sc. in mycology, I have been assigned a task related to mycorrhizal fungi. Is there a webpage or book or paper which lists the species of macrofungi that are known to be mycorrhizal.

Danke!

S.

https://www.agriculturejournals.cz/publicFiles/129_2019-PPS.pdf

Possibly it is var. moroczkovskii (see figure 3 of the paper)

Thank you for the tip regards staining I often stain automatically mounts so good to know about this hint. Accidentally, some spores would not take the stain and do not know if that might avoid adverse reactions on the fibrosine bodies.

Zitat von boccaccio

Hi Steve,

I agree that this should be G. asterum. By the way, I recommend to look at Erysiphales in water and not to stain it with congo red. In some cases, you have to judge if fibrosine bodies are present and I do not know if they survive in congo red.

bjorn

Do you think this powdery mildew on Aster squamatus is a Golovinomyces asterum sl ? Scale is at 2.5um / division.