Beiträge von Steve_mt

Hi, I wonder if someone has the whole collection of these wonderful monographs. I miss volume 7 (pdf)

:coffee:

ITS Results suggested Aspergillus pseudoglaucus and comparing quickly the morphology it should be ok. The second option is C. cibarius.

Polyphasic taxonomy of Aspergillus section Aspergillus (formerly Eurotium), and its occurrence in indoor environments and food

Aspergillus section Aspergillus (formerly the genus Eurotium) includes xerophilic species with uniseriate conidiophores, globose to subglobose vesicle…

www.sciencedirect.com

I have results from ITS sequencing which is suggesting:

1. Ampelomyces sp. (HQ649997)

2. Stagonosporopsis cucurbitacearum (MT312750)

Must be the second one for the presence of Pycnidia

Dear friends,

Today I received several results of ITS sequencing of moulds. This post is specifically about a Cladosporium where the same colony was subculture into two different plates (U11a and U11b), and I submitted both for sequencing. The following results are received (I have fasta / ab1 files for both):

U11a = ok, 100% Cladosporium tenuissimum MK513837, C. cladosporioides MF475952, Hanseniaspora uvarum MG250496

U11b = ok, 100% Cladosporium cladosporioides MT466517,C.cubuliforme MT312738

So I have a tie (undecided) between C.cladosporioides and Cladosporium tenuissimum.

How can I decide which is the one???

1. Morphological examination (can it tell the difference between the two) ?

2. Do other molecular tests (LSU ...???)

3. Edit the sequences and repeat the phylogeny on BLAST/MEga-X

4. Plate on different media?

I prefer the cheaper options (hence avoid doing more tests! unless there are other cheaper options!)

I am an eager amateur

Zitat von thorben96

Hello Hagen,

that doesn't appear to be Penicillium, but you don't show how the conidia are formed.

Because it is important to see whether the conidia are formed in chains or perhaps only individually, but the shape of the conidiophores is also important.

I suspect the conidia are pinched off in long chains.

VG: Thorben

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Fully agree, forget it is an Aspergaceae

After reading some literature, Talaromyces purpurogenus is my best guess. It is used as food colouring. Another clue is that it did not forned red pigments on PDA and I saw that this is so for this species

Safety Evaluation of Fungal Pigments for Food Applications

Pigments play a major role in many industries. Natural colors are usually much safer when compared to synthetic colors and may even possess some medicinal…

www.mdpi.com

My hypothesis is that Penicillium purpurogenum pigment was used in this cooking wine, and some spores might have escaped in the wine. (BTW cheap cooking wine). It used the soaked carton box as a, substrate to grow.

Amazing if this is the case.

When I'm back home from hospital, I carry out some microscopical investigations.

Thanks, but the task is a bit different, I have to mark from a list of 300 macrofungi which are mycorrhizal to be implemented in aforestation etc.

Hi, after my M.Sc. in mycology, I have been assigned a task related to mycorrhizal fungi. Is there a webpage or book or paper which lists the species of macrofungi that are known to be mycorrhizal.

Danke!

S.

https://www.agriculturejournals.cz/publicFiles/129_2019-PPS.pdf

Possibly it is var. moroczkovskii (see figure 3 of the paper)

Thank you for the tip regards staining I often stain automatically mounts so good to know about this hint. Accidentally, some spores would not take the stain and do not know if that might avoid adverse reactions on the fibrosine bodies.

Zitat von boccaccio

Hi Steve,

I agree that this should be G. asterum. By the way, I recommend to look at Erysiphales in water and not to stain it with congo red. In some cases, you have to judge if fibrosine bodies are present and I do not know if they survive in congo red.

bjorn

Do you think this powdery mildew on Aster squamatus is a Golovinomyces asterum sl ? Scale is at 2.5um / division.

Hi , I found an interesting Penicillium s.l. (could be Talaromyces) that was growing on a very specific substrate. Inside my cupboard was an old cartoon of red wine that, tilted and it was slightly leaking. A dull green mould was growing on the wine-soaked cartoon box. I managed to culture a pure colony on PDA, MEA, and CZ

On CZ and MEA it released a very deep red pigment - so amazing!! On PDA no pigment was released.

I wonder if one can help me with the identification (or suggest some literature to consult)

I have not yet examined the material under the microscope as first I wish to have a pure colony to save for the future. The slow-growing mould (at room temp of 29C!) is dull green at the centre, then fading and changes to a cream border and white margin. There are a few options but not sure what it is yet.

I have not cultured that collection from old granulated coffee in a coffee maker, but the species on that specific substrate is Neurospora sitophila, so I was happy with that determination. The one on burnt reeds in the wild might be something different and more important to identify. Could be also N. sitophila but maybe its betterto send a sample for sequencing seeing that I cant find good literature to determine the species macroscopically.

I hope I have not ruined my home Lab because I am reading how a bad contaminant it is -

See here:

https://www.researchgate.net/publication/339105562_Neurospora_sitophila_in_indoor_environment_of_buildings

Yes we are there Neurospora/Chrysonilia (teleomorph/anamorph)

But now I have to get rid of the cultures asap:

"In the school or university laboratory where many students may be cultivating fungi at the same time these fungi can cause lab-wide epidemics, ruining every culture they contact. The best strategy is to discard all rapidly growing, pink, powdery cultures without opening them"

I don't think we will get farther than the genus name regards identification from a morphological approach unless there is some paper dealing with this in detail (I have not found anything good so far). GenBank is also overwhelmed with Neurospora accessions !!!

Thanks for your help, if you find further info on this , please share

Zitat von thorben96

Hi Steve,

very good preparation.

In your new pictures you can see nicely the formation of the conidia.

My first attention of Nematogonum is wrong and isn't similar.

Zitat von Steve_mt

OK folks, I think I have narrowed it down to Neurospora! I see what literature I can find on the genus (or related genera)

I think it is a good direction to look in Neurospora or similar genus.

In "The Genera of Hyphomycetes " i found the genus Chrysonilia with which you can also compare.

best regards,

Thorben

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This is relevant

" David Perkins (Stanford University)

made global collections of Neurospora
species, mostly from burned vegetation,
and developed a method of identifying
the wild collected strains based on unambiguous fertility tests with specially
developed tester strains"

"

Ramesh Maheshwari’s group at the Indian Institute of Science, Bangalore was
the first to have systematically unravelled the Neurospora life cycle outside
the laboratory, in a sugar-cane field2

"

OK folks, I think I have narrowed it down to Neurospora! I see what literature I can find on the genus (or related genera)

Now with regards to the sporangiophores/conidiophores, I fail to see specialized organs. The rounded spores are arranged on the mycelium as a branched arbuscular form. I could notice this by means of some growth on the plastic lid of the Petri dishes. In other instances, I could see special narrow terminal mycelia which had repeated serial constrictions and possible the result of the rounded conidia. Sometimes these are seen as short chains since the constrictions leading to separation are sometimes not consecutive (one after the other one by one) but simultaneous as a group.

Hope this new observation can lead us to somewhere!

thanks thorben

Good morning. I had time to study this pink fungus and the first surprise was its very fast growth on some agar. The fastest growth was on Oat meal agar, then slightly less on Malt Extract and PDA, not abundant on Yeast&Mould Agar and almost nothing on Czapek DOX. The growth on Oat Agar in just three days was impressive!!! The growth was also peculiar, there was rapid vegetative filamentous growth at the central parts of the plate then sporulation took place at the rim and even outside the plate in gross amounts, as if induced by air. I haven't experienced anything like that before.

The cultures have an apple-like fruity scent which is somewhat pleasant.

Hi Thorben.... that's a very interesting suggestion and I will study a bit more your suggestion. Many thanks! The budding of propagules (also seen in the link you provided) really made me believe it was a yeast fungus, and then, there is even a group of pink yeasts.

I have inoculated some plates so I will report that too.

The cylindrical particles seem to cut off from the tips of the main mycelium (Blastogenesis?) but not sure about the rounded propagules. They seem to bud off singly from any part of the mycelium body ?I I will study a fresh mount under the microscope tomorrow. I apply less force on the tape.

Somehow, I didn't see the condiogenesis as in Nematogonum under the stereomicroscope (like the pic below) but I will recheck more carefully tmrw.

Below is an image in the x100 magnification maybe it is helpful. There are millions of these rounded spores but I cannot see a distinct conidiaphore

Enhanced image try to show if rounded spores are budding off from mycelium. They do bud fro meach other!

Another instance which seems to show budding (but it maybe just a spore resting on the edge of the main mycelium ?!)

Probably I won't get any identifications for this one, but it is a very beautiful encounter of a pink-coloured yeast fungus growing a week after a dense population of reeds was on fire. Furthermore, this was found during the arid and dry summer here on our Mediterranean Islands. I pinned it down to Rhodotorula sp. and I am cultivating it on a few agars. I don't know if you can suggest to me some literature about it. Very beautiful, and I could not stop taking pics both in situ and under the microscope.

Hi, I would like to know where I can buy agar media, such as PDA, Czapek Yeast Autolase, Sabourad, Yeast Mold Agar, etc from Europe. The local shop is not very reliable, often needs to import and comes at a price. Once there was an online shop in Germany but I could not remember its name. On Amazon there seems to be unbranded products.

Thanks for helping!

I am studying a Penicillium from indoor environment (probably a common species) which is greenish with dull hue but often turns veige or light brown with age (or high room temp ?) on PDA. On Yeast Mould Agar (I start loving this medium!) they are always with a beige hue. It often forms concentric growth rings.

first of all, I am not sure if I have a pure colony or not. In one of the plates, it seems there are two types of Penicillia!

The one with broad Metullae is much more prevalent and is the one I am studying.

The colonies appear to be homogenous and consist of one species only. I am thinking we are around P. digitatum or P. italicum or P. expansum... but more research is required. Any comments are welcome.

Hi, I wonder if there are people in this forum that are interested in moulds in general. When the summer is to hot for macrofungi, I often switch to moulds. Here I isolated (hopefully in pure form) a mould that is white-cream superficially, without any arieal conidiophores, but forming numerous blackish-brown perithecia (?) or globular structure, 100-200 um across, which give rise to conidia-? asco-? spores. These are cylindrical with obstuse rounded ends, sometimes slightly bent, 8 um approx. I wonder if it is or related to Colletrichium. The colonies look pure (I have some 4 isolates) but under the microscope there seems to be two types of mycelium (!!!).

I anyone can help, even vaguely, I would be grateful.

Eurotium herbariorum (Aspergillus glaucus)

Eurotium herbariorum ( Aspergillus glaucus ) Mould Note:   This fungus has both a sexual and asexual means of reproduction.   When...

thunderhouse4-yuri.blogspot.com

Bingo!!!

I have an interesting and colourful mould, possibly an air contaminant or from debris of plants which I was studying.

It forms a mustard-yellow colony with a broad white border and a darker brownish centre. There are also light greyish-green flocks at the centre. I don't know if I have two fungal organisms intermixed, but two other colonies on other plates formed this pattern.

The reproductive structures are very interesting and there are two types. There are typical aspergillus heads, with a small, slightly swollen vessicle and one series (I think) short phialides. These produce medium-long chains of spinulose, almond to ovate-shaped spores, ca. 5-6 um long. These seem to form the green component of the mould.

Then there are yellow to mustard-brown masses 50-150 um, spherical capsules. The wall (or entirely?) of these capsules seems to be made of 5-6(-8) cellular clumps of cells (sometimes forming a flower pattern or a lobed cloud). I am not sure if this globular body is a 'capsule' or a tight aggregate of these clumps.

What do this story and morphology indicate here please?

Are there Aspergillus species which have two forms of reproduction? Or I have a cultured colony of mixed species. The uniformity of the colonies tells me its one species.

And aahh - there are also coiled structures !!!