Beiträge von Steve_mt

So here are further images and information

-Basidiomycete

-Mycellium intricate gently curved hyphae, 6-8 um thick, septate, congophilous

-Terminal hypha somewhat stains stonger (or there is some myceloplasmic matter)

-Clamp junctions frequent at the terminal septum of the last hypha, large and knobby

-Spores comma-shaped, congophobic (does not stain at all), with (1-)2 small oil bodies, 5um x 2um

-Basidia observed, with distinct dark bodies (oil bodies probably) and (2-)4 sterigmata

Not Sebacina, Not a myxo, Maybe Abortiporus but spores of my specimen are too narrow for A. biennis

!

comapred to here https://www.bioimages.org.uk/html/r145676.htm?15

I saw something similar on the net here:

Mushroom things getting serious after some rain, Oct. 2013

No preamble! Go! I had seen something at the base of a huge old oak on a rural road, and thought it was a clump of dead leaves on a fallen b...

mycologista.blogspot.com

Tnx! I'll take a break from what I am doing and do its microscopy now! This is 10cm x 15cm approx,

Dear friends, I have found this strange pore-bearing flattened (crust) fungus, which is not Sebacina as was my first impression, but later I noticed pores and so it is something else and I was thinking about Abortiporus sp. (biennis) but I have some concerns, because it does not have those orange droplets, and essentially it is all white (with few parts turning beige). Is there other species matching my finding ? I have a dried specimen if it helps further (hope to trace spores). It had the habit of Sebacina , like a huge amoeba engulfing leaf litter and even climbing on bark of an adjacent tree.

A year later, at the exact spot, there was a Daldinia concentrica growing, so I think the photos are the anamorph of Daldinia - Nodulisporium, which was at the moment of turning into the sexual stage. Some typical yellowish matrix can be seen in my photos too. Just a guess!

Hello Oehrling,

I agree that the habit of this mushroom is a bit slender and your suggestion of Sericeomyces is valid. I saw S. serenus which looks like it (but it has a light brown umbo). It is also has dextrinoid spores with matching spore length, although the Q-factor is 1.6-1.9 and the specimen I found is 1.3-1.6. I will investigate a bit more because both suggested species could be my specimen!

Thank you and all the best for the new year!

Sericeomyces serenus (Fr.) Heinem. 1978

Sericeomyces serenus (Fr.) Heinem. 1978 Tassonomia Divisione Basidiomycota Classe Agaricomycetes Ordine Agaricales Famiglia Agaricaceae Sinonimi Leucoagaricus…

www.funghiitaliani.it

I' ve consulted Melzer key and I am concluding on Psathyrella panaeoloides - it looks alike in many ways so I think it is correct!

I found this Psathyrella s.l. which is likely a new species for me. It has hazelnut-brown pileus with tiny white fibrils on the pileus (veil remnants) which goes beige-tawn when dry (hence hygrophanous). Because of these fibrils I do not think it is a Parasola. Spores sizes: (6.8) 7.4 - 8.4 (8.6) × (4.5) 5 - 5.7 (5.8) µm // Me = 7.9 × 5.3 µm; They have a wide germ pore. Cheilocystidia abundant, not very large / Pleurocystidia seen, rare or infrequent. The fibrils of the remnant veil seems to have some incrustated pigment. I think I have also seen Pileocystidia (not sure - see last image).

Any indication would be appreciated

Is that a real photo or something generated with AI!!!! Amazing image - What camera/Lens was that!

The posted plant looks like Cyperus eragrostis or glomeratus.

Can you provide a photo of the cross section of one fruiting body ?

Large size and robust

Dirty brown center and scales

Yellowing at the base and then fades away after 30mins

Cystidia small and infrequent

Basidia 4-sporous (ca. 30 x 7 um)

Spores 5.4 x 4.1 average

Strong phenolic smell

Habitat under pine trees

Agaricus moelleri (or something else?)

Spores in Iodine solution:

Cystolepiota sp....

I have found this interesting Lepiotoid macrofungus under Quercus ilex (and pine trees closeby) which surprised me for having no dextrose reaction with Iodine, hence questioning if it is a Lepiota, unless there are a group of Lepiota species (or circumcrscibed genus) for non-amyloid Lepiotae.

The fungus is cream-beige overall with carmel-brown veil that splits into scales like a Lepiota does! The stipe is white-pruinose but when touched or with age it is peach-brown, The flesh at the base is dark brown, lighter above. The ring is ascending, white above, peach-brown below

Spores in groups of 2-8 (now this is a Lepiota-thing!) tiny, difficult to explain the shape vut I would say elliptical with one end slightly more flattened than the other, but not distinctly so. They do not stain well with COngo red. Oil body and apiculum present

(3.5) 3.8 - 4.4 (4.6) × (2.1) 2.4 - 2.8 (3) µm

Q = (1.3) 1.5 - 1.7 (1.9) ; N = 35

Me = 4 × 2.6 µm; Qe = 1.6

Cheilocystidia quite frequent, grouped together and clavate to sphaeropedunculate, smallish,

15.74 − 27.39 x 5.27 − 9.7 µm (average 20 x 7 um)

basidia not much in good shape, but 4-sporous, small, with 2.8um long needle-thin sterigmata

The pileus skin is a trichoderm but the veil above is a hymenoderm of sphaeropedunculate shaped elements, quite similar to the cheilocystida, but remarkably larger - 36.9 x 14.1 µm (range: 26.11−42.84 x 11.56−16.36 µm)

Section 2.5-4.2cm high and cap 1.5-2.9 cm wide

Further details on the images

Dear Michael,

No problem, we are here to discuss, share opinions, learn and after all have fun communicating with real people rather than stuck in some AI app or screen. Thanks for the time to reply and have interest in my finding, and same goes to Oehrling, if he is still wants to talk to me after disappointing him a bit with the spore-stain

For now I place it as L. leucothites unless a better option arises, but as you said it looks alike and spore measurements are within the range, although from the literature shown, the range is quite wide!

Cheers!!!

Spore sizes of L. leucothites found on online literature

First nature: 7-9 x 4.5-5µm

University British Columbia: 7–10.0 x 5.0–7.0 µm

Mushroom expert: 8-11 x 5-6.5 µm

I also found a twin brother of my specimen, smallish with ring hanging on the rim of the pileus and no traces on the stipe!!!

Leucoagaricus leucothites (Vittad.) Wasser 1977 media - Encyclopedia of Life

New measurements in water.

9.27 5.77

7.93 5.18

7.23 5.45

8.55 5.08

8.79 6.25

8.28 6.04

7.83 5.36

8.83 6.05

8.00 5.91

7.20 5.37

7.55 5.16

6.45 5.40

8.78 5.60

8.55 5.40

8.82 6.03

6.84 5.49

8.31 5.06

7.36 5.59

8.99 5.30

8.77 6.24

7.45 6.05

7.83 5.60

8.12 5.73

8.05 5.91

7.90 5.72

7.36 4.88

8.16 5.76

7.47 5.29

7.57 5.68

7.77 5.58

6.90 5.00

8.04 5.27

7.38 5.25

7.63 5.34

8.34 5.95

8.28 5.59

7.49 5.46

8.57 5.78

7.99 5.94

9.55 5.46

7.89 5.45

8.94 5.73

8.51 5.65

8.71 5.68

7.67 4.88

(6.5) 7.2 - 8.8 (9.6) × (4.9) 5.1 - 6 (6.2) µm

Q = (1.2) 1.3 - 1.6 (1.7) ; N = 45

Me = 8 × 5.6 µm ; Qe = 1.4

Deeply sorry for not mentioning it - in many images the spores are stained in Congo Red (and also some post editing of the images to increase the contrast and make the bg whitish) and a few in Lugol's iodine to show their dextrinoid character typical of Leucoagaricus.

Without stain, the spores are hyaline.

Some comparisons with Leucoagaricus cinerascens hold well.

https://www.mycocharentes.fr/pdf1/1331.pdf

MycoDB : Fiche de Leucoagaricus cinerascens

Dear friends, I need your advice on this nice Leucoagaricus I found in a park under Pinus halapensis (and Eucalyptus) on calcareous soil. A rather robust basidiocarp, white/beige with a rather pale brown or coffee-milk pileus which in old specimens cracks into large scales. Spores dextrinoid, almond-ovate with rounded end and an indistinct or absent germ. pore (not sure about it to be honest, but seems not evident). The cheilocystidia are quite narrow and many are subcapitate or ventricose. The pilleipellis was also examined and terminal hyphae observed (see images). I am undecided about a few species such as L. leucothites? (must be white and has larger cheilocystidia!) L. wichanskyi (germ pore should be absent?) or L. cinerascens (cheilos are wide?) but I can't make up my mind.

Pileus up to 7cm wide

Spores dextrinoid 8.5 − 12.9 x 5.3 − 7.1 µm (10 x 6 um) / Q-factor: 1.4-2.0 (1.6)

Cheilocystidia subcapitate or ventricose 31.1 − 55.0 µm x 6.2 − 11.4 µm ( 42.28 9.14 µm)

Images attached here

Zitat von Steve_mt

Dear Oehrling and Michael, thank you for your replies.

Ring or not, I am not sure because I do not see a scar on the stem, but you could be right.

I reckon microscopy is required at this stage and I have marked that I have an exsiccatum for SM-552. I report findings over the weekend.

Thank you

LG / FG

Stephen

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So finally I had time to examine the spores and gills of an exsiccatum which was not in very good shape (larvae destroyed physically part of the specimen. Here are some notes:

The gills turned beige-brown with age

Spore print white

Spore deposit/production abundant

Cystidia generally absent (but check image of a possible finding of a cheilocystidium)

spores broadly ovate or shield like with apiculum and distinct germ pore

Sizes:

(7.5) 7.8 - 9.3 (10.2) × (5) 5.3 - 6.1 (6.6) µm //

Me = 8.5 × 5.8 µm; Qe = 1.5 ; Ve = 147 µm3

Ring may be present and got detached and hanging at the rim during basidiocarp growth

I am thinking on Leucoagaricus, specifically L. leucothites ?

>I cannot upload pics!!!<

.... wanted to add that I bought this

https://proficook-france.fr/en/proficook/37-deshydrateur-alimentaire-4-ebenex-proficook-pc-dr-1116-4006160501117.html

The lowest temperature is 40C and unfortunately my thermometer (external metal thermometer) indicates 44C eith lid closed. Removing the lid and raising some compartments, I get around 36-40C. Hope this is fine for molecular testing and cell conservation for microscopy.

I wonder if anyone uses this too

This topic is of interest also to me. At the moment I have been struggling in finding a cheap or free image stacking software mostly for myxo but also for micro-images and small mushrooms. Any suggestions ?

Question 2 is that I have an SLR and I can have an adapter so as to fit in the microscope trinocular head (an old 1980s AxioLAB Zeiss). Do you have experience if photos taken this way produce good quality images. I am currently taking pictures of my eyepiece from an expensive point and shoot camera. Its 4x optical zoom enlarges the image nicely and it is very fast.

With the SLR the shooting is a bit slow procedure as I assume one has to refocus and adjust settings between live view and switching to shooting mode. Also I loose the ocular micrometer option.

Dear Oehrling and Michael, thank you for your replies.

Ring or not, I am not sure because I do not see a scar on the stem, but you could be right.

I reckon microscopy is required at this stage and I have marked that I have an exsiccatum for SM-552. I report findings over the weekend.

Thank you

LG / FG

Stephen

I am trying to identify this species found under carob trees and some pine needles from a tree further apart, on damp humus. No partial veil and I don't see a vulva. 26/11/2020