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Images of Veil (Suprapellis), Pileipellis and stipe hyphae (caulocystidia ?)

Dear friends, following Olivier tip, I have examined the spores, Cheilocystidia, Pleurocystidia, Caulocystidia, Basidia, Suprapellis (veil?) and Pileipellis of this interesting specimen. So here is the data in several posts.

Spores, tawny brown, almonds to phaseoliform, apiculus distinct, globular, gem-pore probably absent, surface smooth or finely pitted (?), oil bodies not seen. size:

(8.9) 9.4 - 11.9 (12.2) × (5.5) 5.7 - 7 (7.7) err

Q = (1.4) 1.45 - 1.9 (2) ; N=31

V = (151) 172 - 287 (331) err3

Me = 10.4 × 6.4 err ; Qe = 1.6 ; Ve = 226 err3

basidia

Clavate with broad ends, (x2-) x4 sterigmata, each 2.5-3.5 um long, slender horn-shaped, size:

(31.6) 32.5 - 38.6 (39.9) × (8.6) 10.3 - 11.3 (12) err

Q = (2.8) 2.9 - 3.6 (4.5) ; N=7

V = (1469) 1975 - 2364 (3001) err3

Me = 36.4 × 10.7 err ; Qe = 3.4 ; Ve = 2188 err3

Cheilocystidia abundant, lining the entire lamella edge, narrowly utriform with crystals on the apex, sitting on a short foot cell (septate base), hyaline, smooth, size:

(48.1) 50.4 - 67.7 (68.4) × (12.5) 14.6 - 17.2 (18.4) µm

Q = (3) 3.1 - 4.65 (4.7) ; N=13

V = (4788) 5656 - 9115 (10756) µm3

Me = 56.4 × 15.6 µm ; Qe = 3.7 ; Ve = 7240 µm3

Pleurocystidia frequent, same as cheilocystidia perhaps more slender and slightly longer

Suprapellis (veil?) composed of elongated cylindrical hyphae, 6-8 um wide, hyaline (unpigmented), with the terminal element being curved (flexuose) and unswollen rounded tip.

Pileipellis much more broad, sausage-shaped or broadly cylindrical, with distinct brown pigments encrusting the walls,

(32.4) 34.8 - 63.3 (73.4) × (8.8) 10.5 - 16.5 (18.9) µm

Q = (2.1) 2.4 - 5.1 (6.3) ; N=20

V = (1838) 1955 - 7265 (7655) µm3

Me = 48.5 × 14.1 µm ; Qe = 3.5 ; Ve = 5192 µm3

Caulocystidia (and hyphae of the stipe) tall and narrow cylindrical shaped, smooth, hyaline, straight, 7-11um wide ( 45-100 um long) with occasional swollen and almost globular (balloon shaped) terminal hyphae (=caulocystidia?) measuring 25um long and about 15um in width.

Clamp connection examined in all tissue explained, rather large sometimes.

Very small fruting body (1cm) , not sure if it is the normal size, cap very fibrous - Inocybe sp. ?

Sample collected

Hi, I found this black cup fungus (Ascomycete) and I considered Smardea and Pseudoplectania but now I am fixed on a dark-coloured Peziza sp. This population growing in mossy ground under Pinus halepensis in a forest path, in calcareous soil. In sunlight, the ascocarp has a purplish-brown tinge. I am showing this collection from photos I have taken in the field. Further examination in the Lab follows shortly.

I will produce images of spores, cheilocystidia (and pleuro) and pileipellis today. If other features are required I let you know. (The english translation of the fungus you suggested is "Cadaver"!!!)

Thank you so much for these helpful notes. I was away from mycology for two days but today/tmrw I should do some investigations. The fruiting body has a hint of blue. The stick was found around and probably is of Pinus halepensis. I consult Bernnichia's book . I keep you posted. tnx.

Can this small pore-bearing fungus (Polyporales ), approx 1 cm wide, be identified. The pores are very minute, barely visible with the naked eye. They were attached to a stick and I think they were facing down (hence not resupinate as the pic may indicate).

Hello, I found this nice species of Inocybe growing from leaf litter of Pinus halepensis and I think (not sure) to correspond to Inocybe rimosa (=I. fastigata). The colour, cracked cap and fluffy stipe corresponds. Wonder if you have different opinions or maybe there are other newly described species within the rimosa group.

LG Steve

The images are amazing. Do u have the monograph ? If not I can send

Thanks, Clavaria for the info.

I reworked the spores and the sizes are those below, making it a clear D. coprophila (spore size = mean 12.4 x 7.6)

(10.4) 11.8 - 13.2 (14.1) × (6.2) 6.9 - 8.2 (9.3) err

Q = (1.5) 1.51 - 1.8 (1.9) ; N=31

Me = 12.4 × 7.6 err ; Qe = 1.6 ; Ve = 381 err3

Zitat von Ingo W

Hello!

Zitat von Steve_mt

Clitocybe it is. I think it can remain within the variability of C. hobsonii!

The real name is "Clitopilus", not Clitocybe.

http://www.pilzflora-ehingen.d…ora/arthtml/chobsonii.php

VG Ingo W

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Dear Ingo,

Yes I got tired yesterday - I corrected the names. I agree re hobsonii, yesterday night I found the differences between the two, mainly the spore length with hobsonii being: 7.5-8.5 and daamsii : 8.0-11.5 um. I really a merry-g0-round here but it is nice to learn. i will never forget the vertical ridges now

Thanks to all - I appreciate a lot

LG

Steve

I abandoned C. hobsonii because of its smaller spore size. Also, the robust apiculum is something which mislead me. But yes, I agree about the longitudinal shadows of the spores (see a better image here!). Moreover, I went to check for the Cheilocystidia and could not find any -I think what I am seeing are basidioles. If I remember what I read in the afternoon, lack of cheilocystidia is a Clitocybe character.

Clitocybe it is. I think it can remain within the variability of C. hobsonii!

Further research and applying the key on European species, I have landed on Crepidotus subverrucisporus , typically on calcareous habitats

https://www.mykoweb.com/Crepidotus/species/Crepidotus_subverrucisporus_subverrucisporus.html

C. variabilis has smaller spores (Sp 5.5-7.5 x 2.5-4 μm), and more narrow - subcylindrical

C. cesatti has more broad (subglobose) spores (Q. <1.4)

C. luteolus has yellow tinge (at the less)

C. lundellii* is closely related but it is said to have brownish colours when dry and not calcareous

So at the moment, this is Crepidotus subverrucisporus or C. lundellii - I guess I have to re-examine the cheilocystidia?

C. lundellii: https://www.hlasek.com/crepidotus_lundellii1en.html

* One of its synonyms is Crepidotus inhonestus !lol! - why this fungus is dishonest haha!

I have given a quick check at the spores and I think you are right about Crepidotus - the spores are too large for C. hobsonii, and their shape, a bit like a lemon due to a distinct-swollen apiculus is also Crepidotus rather Clitocybe.

Spores:

(7) 7.1 - 8.3 (8.9) × (4) 4.1 - 4.8 (5.2) µm

Q = (1.5) 1.52 - 1.8 (2) ; N = 12

V = 61 - 98 (110) µm3

Me = 7.6 × 4.5 µm ; Qe = 1.7 ; Ve = 81 µm3

I think you are correct (or Botryotinia) but it must be a species that does not have a branched conidiophore. I always associated Botrytis with a branched conidiophore (that is I have always seen B. cineraria). I see what I find in this genus with large spores. B. elliptica for example!.

Thanks for the moment !

Zitat von thorben96

Hi Steve,

compare with Botrytis sp.

best regards,

Thorben

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OMG there is also subcoprophila! How to tell the difference between the two? I have some microscopic data at hand, such as the spores.

Deconica coprophila on horse dung pellets - are all four the same species ? Maybe the third one is something else ?

Thanks

I forgot to add that I managed to get a pure colony on Sabourad Agar. It formed a white or light grey mycelium without quick fruiting bodies. These were formed after 1 week when the mycelium covered the whole plated and they were formed at the walls of the petri dish (drier regions ?), somewhat not that black and shorter. Now I have inoculated OAT, PDA and CZapek to see if it helps. Really wish to learn this microfungus. Chloridium is close but spores are larger 10-17 um long, 8-11 um wide

Growing on the pulp of a fallen fruit of Hawthorn (Crataegus monogyna) was a dark mycelium with white heads which took my curiosity to investigate. Under the microscope I could see a simple, dark-coloured (blackish-brown) septate conidiophore (somewhat paler at the tips or undeveloped ones) with a small head-like cluster of hyaline-white conidiospores. These are liberated readily in water or soap water, so I suspect that there is some slime involved. However, at the location where the conidia are present are 3-6 small, flattened plugs projecting out onto which clusters of spores are attached. The spores are subglobose to oval, variable size, possible not hyaline but opaque since spore contents (eg oil bodies) are not seen.

Hi Thorben, I have found something similar, and I could not pin down the genus, hence posting soon on this forum. Unfortunately, I don't think we have the same organism because mine grows on decaying fruit and the spores are not so hyaline as they dont show the oil bodies.... but is not so different in general. Do you have some key to the genus ?

Thanks for trying to cheer me up! I am a very emotional Brazilian fan, and I need 3-4 days to be back in the mood. Tomorrow I prepare mulled wine to join you in spirit! (...and do some work on this pluteus).

It was nice to see again this tiny orange fungus - Pithya cupressina on fallen leaves of Cupressus sempervirens. (Malta, 10-12-22).

Thank you Harold - later I get grips on Flora Agaracina and dive into the keys. I keep you posted after I recover from mental trauma after my all-time fav. football team got eliminated from the world cup so dramatically - Brazil. I guess many Germans here have passed this already.

I am working on another new basidiomycete, this time a clear pluteus for its free gills (distant from the stipe), rose-light brown spore print and lack of veil, ring and volva. The light lemon-yellow colours of the stipe and the subspherical spores indicates a P. romelii, but the cream with a very faint hue of yellow colour of the pileus is quite off for the species (P. romelli is caramel to cinnamon brown). So here I am discussing this fungus with you.

Basidia oblongish-subclavate, sometimes flexuous, predominantly 2-spored, rarely 3-spored, up to 35um long

Cheilocystidia are large, lageniform to broad utriform (prone to correction!)

Pleurocystidia present, similar to the cheilocystidia, perhaps a bit smaller

Spores subspherical (some looks spherical) to globose-ovate, Me = 7.1 × 6.3 µm; Qe = 1.1; Ve = 152 µm3; 5.9) 6.2 - 8.1 (8.6) × (5.2) 5.7 - 7.1 (7.7) µm (n=33). In lugol's iodine they showed a dextrinoid reaction, while in Cotton Blue (with lactic acid), the spores collapsed inwards.

They were growing from leaf/twig litter of Eucalyptus plantation. I had in mind Pluteus romellii s.l. and Pluteus boudieri P.D. Orton 1960

I am aware by the work of : Holarctic Species in the Pluteus romellii Clade. Five New Species Described and Old Names Reassessed [ https://www.mdpi.com/2309-608X/8/8/773 ] but all their described species do not show this pale pileus. P. boudieri is my best match so far.