I was wondering if at least we can give it a genus name: Cystolepiota sp.
Beiträge von Steve_mt
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Good Morning. From my archives of unidentified fungi, here I am showing a tiny fungus, barely 4 mm tall and 2mm wide growing on decaying bark of Ceratonia siliqua (carob). Micro is not available since I have neglected this collection to spend time on others. It can be an abnormally small fungus of some common species, like a Psathyrella sp. (melanthina?) or whether it is the normal size of a fungus that I don't know at all !!! In desperation, I tried googling 'tiny brown mushroom' but I got a bunch of Panaeolina, Conocybe and Mycena and unrelated searches. == twitch
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P.s. the images of your micrographs should be cropped to out give more detail, like Steigerwald mushrooms image
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I find this post interesting as I was working with a species of the grisinea group (under Qurecus ilex) in calcareous soil, resulting in a parazurea (or orchospora?) which had a rather colourful spore print (not cream or magnolia, but close to the flesh of a peach (ochre in English), strong but short reticulations and much dull colours of the pileus. So what I am trying to tell you is that u can exclude parazurea.
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I am seeking help for a fungus (only 2 specimens in situ) on decaying wood (Carob branch) in a damp habitat. It was very small, with its pileus about 1 cm across and stipe 3 cm tall. Pileus (and less so the stipe) was covered with a fine felty (tomentose) coat smf the margin was finely appendiculate. The problem is that its microscopy was boring (or unsuccessful
)First of all I could not detect good spores , yet I think they are small, 3-4um rounded to broadly ellipsoid bodies. The cystidia if present are not specialised and maybe I was seeing basidiomes. The only successful microscopic feature was the pileipellis, intricate hyaline elongated parallel-running hyphae that are smooth and quite long, terminal cells unspecialised too. I think I have seen clamps in the pilepellis. (see image 8129). This is just my interpretation of what I am seeing and maybe wrong, but the physical characters might be good enough
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What would be the species name of the 'Rotfuss' ? Xerocomellus chrysenteron ?
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Just wanted to say lovely photos both the macro and the micro (I am just amazed at the clear background too!) . I scroll back to see them another time! Cheers
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He did but with handshake
.Another option a I told him is that the young specimens had remnant veil and got lost with time-transport and hence his specimen might as well be a Coprinopsis.... but for me the pleated shape of the pileus with grey furrows and tawny-brown ribs is indicative of Parasola! -
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I received an exsiccata collected on the 20-Sep-2021 from a close friend/botanist who found it in a garden and growing close to a stump of a small tree. This was growing during a warm season here in Malta. After initial examination, I am sure that it is a Parasola species. However narrowing to species level is quite uneasy.
First of all, a strange character is the presence of pubescence especially at the base of the stipe (I am used to glabrous stipes for Parasola). Next the pileus lacked the typical thick-walled setae (see images) but there was some kind of hyphae forming free fibrillose tufts on the pileus. The pileus was ribbed, with the ribs being brownish and the furrows being grey. The exsiccata has lost its basidia and most cheilocystidia since the edges had eroded/deliquesced, but I managed to see two large spherical bodies which might represent cheilo-or pleurocystidia, but since being globular, they might be something else. The pileipellis consisted of not very long rectangular to cylindrical hyphae, with truncated ends, sometimes a small branch (foot) and with some pigmented (?) incrustations. The following sizes were measured:
37.1 [56.4; 75.8] 95.2 × 12.6 [17; 21.5] 25.9 µm
Q = 1.5 [2.8; 4.2] 5.6; N = 9; C = 95%
Me = 66.1 x 19.3 µm; Qe = 3.5
The spores are ovoid or almond shaped (depending the plane of view) with a distinct germpore
7.4 [8.8; 9.3] 10.7 × 5 [5.7; 5.9] 6.6 µm
Q = 1.3 [1.5; 1.6] 1.8; N = 40; C = 95%
Me = 9 x 5.8 µm; Qe = 1.6
Caulocystidia or hairs looked as elongated shortly-cylindrical hyphae
After spending considerable time researching, I am seeing P. lactea and P. kuehneri as the best options especially for the small size of the spores.
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Yesss!!!! I found it - does exactly as I wished
Thank you again!
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Hi guys, a quick question. I wish a handy shortcut link (or two) that will list for you all the posts you created (and a second of all the list you participated in) for easy access. Maybe this already exists and I am not aware of it. I guess it would be in the member profile area, or even in the title bar!
Thanks and well done for this precious forum and the wonderful experts and friendly mycophiles/contributors.
Steve
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Distribution very south and north african!
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This should be Xerocoprinus arenarius in a grassy patch in a garigue/steppe habitat in calcareous soil. Note the root and the large pore in the spores. Dec 2020, Island of Gozo, Ma lta
13.8 [15.4; 16.1] 17.7 × 8 [9.3; 9.8] 11.1 µm
Q = 1.5 [1.6; 1.7] 1.8; N = 31; C = 95%
Me = 15.8 x 9.6 µm; Qe = 1.7
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Hello Steve!
That was exciting to follow from the first find till scientific publication. Congrats!
Thanks for your kind words - yes like a diary!
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Dear friends,
I and my supervisor have just published this paper about Cortinarius ayanamii, an understudied species from the Mediterranean region forming mycorrhizal association with Cistus monspeliensis (and C. albidus) corresponding to the first Cortinarius for Malta. I think here is the right place to share. Many thanks to Giuseppe Venturella, Uwe Winkler, Pablo Alvarado and Dimar Balint who were involved in this paper.
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I measured another set of spores with the following results:
10.1 [11.4 ; 11.9] 13.2 × 5.6 [6.5 ; 6.9] 7.8 µm
Q = 1.4 [1.7 ; 1.8] 2 ; N = 41 ; C = 95%
Me = 11.7 × 6.7 µm ; Qe = 1.7
On Inocybe.org (well done for the work) I read :
Spores up to approx. 12 (13) µm, often drawn out at the apex
I did not manage to PM you (I have photographed the dry specimens) so if you want to send me an email: info [at] malta wild plants .com (remove space and brackets) I can comm re sending samples
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The collection is from the Maltese Islands and it it was collected on December 2020, but working on the determination this week. I can only supply dried basidiocarps - is that still ok to send ?
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Thanks Ditte, I was pretty sure that I was in the right group but I am no authority or have much experience with Inocybe. So I thank you for helping me out.
From the Italian website about I. nitidiuscula :
Stipe: " Da 4-6 (7) cm, cilindrico, spesso ricurvo alla base dove appare un poco ingrossato, senza mai formare un bulbo vero e proprio, delicatamente decorato da fibrille longitudinali per quasi tutta la sua lunghezza, pruinoso solo nella parte apicale , di colore ocra pallido con sfumature pink-rossastre specie in età adulta . " I interpreted that the what I am seeing is delicate longitudinal fibrils almost towards the entire stipe , while pruinosity is lmited below the cap at the upper part of the pileus.
The collection is from the Maltese Islands.
Unless some accident occurred, I have dry specimens to 1) check some feature and 2) to send you for your own examination. (PM yr address). I will tomorrow check the spores again, but I am quite sure they are correct.
I took images of the spores in KOH as well I just took in Iodine to test amyloid reaction. The measurements must be good but I can recheck.
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I am thinking Inocybe nitidiuscula (Britz.) Saccardo for:
Redenning / browning of stipe in adult specimens, Spores match, habitat under pines ok, ornamented with tufts till the base, pruinose at the upper part
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I am working on the ID of an Inocybe with cracking and small (2-3 cm across) pileus with chestnut color and variable striated with beige bands or patches (maybe because of hard soil over) growing at edges or under of a pine afforested area.
My options are short listed on fuscidula / nitidiuscula / leiocephala. The spores are broadly ellipsoid navicular (front view), almond-shaped (dorsal view), 10.6-13.2 um x 5.2-7.6 um (mean12.1 x 6.6) with a Q of 1.8 +/- 0.3. The cheilocystidia have a thick wall (not extremely thick) with apical crystals, in clusters 60-70 x 13.5-18um, while the pleurocystidia are more or less similar but not frequent and usually solitary.
Pileipellis:
Intricate cylindrical hyphae of various widths, but apparently two types, one that are cylindrical and slender, hyaline, 5 um wide, irregular and intricate, and the other type formed by larger and broader (11-14 um wide), sausage-shaped hyphae with pigmented incrustations on the walls.
The stipe seems to redden a bit in old specimens otherwise ocher-cream when fresh and undisturbed. The pruinosity seems to run half the stipe length (differs between various specimens) in some it seems going down in a patchy distribution below the lower half of the stipe.
I have a whole set of characters that I can show and discuss, but I think the above is the crucial. Photos attached.
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I have a question related to the cultivation of molds on media and selective media like PDA, MEA, OAT Agar, Czapek, etc. I have left some 25g of antibiotics (approx. 10g Augmentin, and 14g Moxiclav *). This was for human consumption but they are general antibiotics. I want to add them to my media to control bacterial colonies. [* both brands state that each 1g tablet is composed of 875g Amoxicillin + 125g Clavulanic Acid]
Q1: Can you suggest how much I should put, say in 250 ml of media during preparation.
Q2: Is it Ok to add when hot, or hot water destroys the antibiotic?
Q3: They expire end 2021 but do they really expire for in-vitro use?
